Precision biomolecule processing for single-molecule technologies

Project Summary

The study of complex biomolecules often relies on selective tagging, enrichment, concentration, or barcoding before measurements. Single-molecule analytical techniques, such as nanopore sensing, could be greatly enhanced by these preparatory steps but remain constrained by the limited tools currently available. Efficient and versatile chemical strategies will open new possibilities for studying the natural complexity of biomolecules.

In this project, candidates will develop new reagents and catalysts for processing biomolecules with high efficiency and selectivity, thereby facilitating their readout with advanced nanopore systems. State-of-the-art experimental and computational approaches will be applied to discover and optimise new chemistries through the screening and evolution of libraries of small molecules, proteins and nucleic acids. Advances from this project will both underpin next-generation analytics and diagnostics, and enable the interrogation and manipulation of an expanded range of biomolecular targets.

Advisor:

• Professor Yujia Qing (Senior Group Leader, EIT & Associate Professor of Organic Chemistry, Department of Chemistry, University of Oxford)

Skills Recommended

• A 4-year undergraduate degree (or equivalent) in a relevant scientific discipline (e.g. Biochemistry, Biology, Chemistry, Computer Science).
• Research experience, with skills in laboratory techniques and data analysis, ideally in areas such as protein biochemistry, molecular genetics, or organic chemistry.
• Strong self-motivation and enthusiasm for research in a dynamic and evolving field.
• Clear and effective communication skills, both written and verbal, with an interest in working across disciplines.

University DPhil Courses 

• DPhil from the University of Oxford

Relevant Literature

• Bo, Z., Lim, Z.H., Duarte, F., Bayley, H. and Qing, Y. Mobile molecules: reactivity profiling guides faster movement on a cysteine track. Angew. Chem. Int. Ed. 135, e202300890( 2023).
• Lan, W.-H., He, H., Bayley, H. and Qing, Y. Location of phosphorylation sites within long polypeptide chains by binder-assisted nanopore detection. J. Am. Chem. Soc. 146, 24265-24270 (2024).

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